This protocol describes gating strategies to study T cells in mouse thymus, spleen, and blood. We outlined different T cell populations based upon extracellular and intracellular markers. Through the incubation of samples Using the antibody blend for extracellular staining, put together all necessary buffers to detect intracellular cytokine generation in https://kaiserlichesinterieur.de/2024/04/29/entdecken-sie-die-kraft-der-liebe-6-moglichkeiten-mehr-liebe-in-ihrem-leben-zu-kultivieren/